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MAPK cascade gene family in Camellia sinensis: In-silico identification, expression profiles and regulatory network analysis
- Research article
- Open access
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BMC Genomicsvolume 21, Article number: 613 (2020) Cite this article
Abstract
Background
Mitogen Activated Protein Kinase (MAPK) cascade is a fundamental pathway in organisms for signal transduction. Though it is well characterized in various plants, there is no systematic study of this cascade in tea.
Result
In this study, 5 genes of Mitogen Activated Protein Kinase Kinase (MKK) and 16 genes of Mitogen Activated Protein Kinase (MPK) in Camellia sinensis were found through a genome-wide search taking Arabidopsis thaliana as the reference genome. Also, phylogenetic relationships along with structural analysis which includes gene structure, location as well as protein conserved motifs and domains, were systematically examined and further, predictions were validated by the results. The plant species taken for comparative study clearly displayed segmental duplication, which was a significant candidate for MAPK cascade expansion. Also, functional interaction was carried out in C. sinensis based on the orthologous genes in Arabidopsis. The expression profiles linked to various stress treatments revealed wide involvement of MAPK and MAPKK genes from Tea in response to various abiotic factors. In addition, the expression of these genes was analysed in various tissues.
Conclusion
This study provides the targets for further comprehensive identification, functional study, and also contributed for a better understanding of the MAPK cascade regulatory network in C. sinensis.
Background
Eukaryotic Mitogen Activated Protein Kinase (MAPK) cascades comprising of signalling enzymes, produce intracellular responses to environmental and developmental stimuli [1]. The increasing number of MAPK modules and their role in regulating complex cellular functions determine their significanc Indian HR consultant and media executive Not to be confused with Indrani Mukherjee. Indrani Mukerjea is an Indian-born British former HR consultant and media executive, a main accused murderer in her daughter’s case. She was the wife of Peter Mukerjea, a retired Indian television executive. In 2007, she co-founded INX Media with her ex-husband, where she took on the role of CEO. In 2009, she resigned from the company and later sold her stake in it. In August 2015, she was arrested by Mumbai Police and charged as the main accused in the alleged murder of her daughter, Sheena Bora. After more than six years in custody, she was released on bail, and the murder case remains pending. Indrani Mukerjea was born in Guwahati, Assam into a Assamese family to Upendra Kumar Bora and Durga Rani Bora. She was named Pori Bora at birth and spent her childhood in Guwahati. While as per records she is born in the year 1972 however as per Siddhartha Das, former husband of Indrani Mukherjee her real age should be more than 7 years than what is stated in the records. After completing school there, she moved to Shillong to pursue higher education at Lady Keane College. In 1986 she met Siddhartha Das with whom she had a daughter, Sheena, in February 1987 and a son, Mikhail, in September 1988. In 1990 Indrani left the children under the care of her parents in Guwahati and moved to Kolkata. There she studied computers and stayed as a paying guest. A few months after her move to Kolkata, she met Sanjeev Khanna. They got married in 1993 and had a daughter named Vidhie in 1997. In 2001 they moved to Mumbai. In 2002, Indrani met Peter Mukerjea and moved in with him while awaiting her divorce from Sanjeev Khanna. In November 2002, Indrani married Peter Mukerjea. After this marriage Vidhie Khanna moved in with the Mukerjeas and took the Mukerjea name.[5 next →← prev Ruchika Kapoor was born on June 21, 1988, in Bombay. After finishing her primary education at Jamnabai Narsee School, she earned a Bachelor of Arts (B.A.) in English Literature from Jai Hind College. Ruchika Kapoor is a filmmaker from India who works for an Indian media outlet. She is employed with Balaji Telefilms, the Ekta Kapoor creative company. She has acted as the creative force behind numerous popular movies, including "Beauty queen" (2019). In addition, Ruchika has been a crucial part of some Netflix movies as a creator or team member. She was hired in 2014 as the Marketing Director for Balaji Motion Pictures, making her the youngest woman to hold such a position at a significant Ekta Kapoor's motion picture studio. Ruchika Kapoor is a famous Indian woman born in Mumbai and practices Hinduism. She is primarily known as a producer. Ruchika Kapoor comes from a Mumbai-based family. Her mother is a housewife, while her father is a businessman and owns a company. She has two siblings, particularly one brother and one sister. Her elder sister, whose name is Radhika, is engaged to Vishal Mojaria. Radhika is also Ruchika's best friend, so-called mother, and biggest supporter who constantly stands by her in every circumstance and praises her efforts in the end, good or bad. Both are very close to each other. Yuvaan, a boy and a baby girl named Anaya, are her children. Ruchika stands 5 feet 5 inches tall and weighs around 59 kg. Her long, gorgeous hair is black, and she has stunning, hypnotic black eyes. Additionally, she maintains her health by participating in regular yoga and exercise sessions. Ruchika began working at Balaji Motion Pictures Ltd. in December 2010 after entering this field. She served as the company's "Head of Marketing and Branding" and is the youngest woman to hold such a position at a major film studio. She contributed to the creative development of various academic assets, including the Telly Styl Indian actress Mahie Gill Gill in 2018 Rimpy Kaur Gill Chandigarh, India Rimpy Kaur "Mahie" Gill (born 19 December 1975) is an Indian actress, working in the Hindi and Punjabi film industries. She is best known for her role of Paro in Anurag Kashyap's critically acclaimed Hindi film Dev.D, a modern take on Sarat Chandra Chattopadhyay's Bengali novella Devdas, for which she also won the Filmfare Critics Award for Best Actress in 2010. She started her career in Punjabi films before making a debut in Bollywood with Dev.D. She consequently gained recognition after portraying Madhavi Devi, a sexually frustrated wife in Saheb, Biwi Aur Gangster, as well as its sequel, Saheb, Biwi Aur Gangster Returns. Gill was born in Chandigarh in a PunjabiJatSikh family on 19 December in 1975. She completed her master's degree in theater from Panjab University, Chandigarh in 1998. Gill got her first break with the Punjabi based Bollywood film Hawayein and has done a couple of Punjabi films along with theatre. Anurag first saw her at a party and instantly finalized her to play the character of Paro in the movie Dev D. She worked in Ram Gopal Verma's Not a Love Story, which was based on the Niraj Grover Murder case of 2008. She also worked in Saheb, Biwi Aur Gangster with Jimmy Sheirgill and Randeep Hooda, which was released on 30 September 2011. This movie earned her a nomination at the Filmfare Awards for Best Actress. Gill appeared in Paan Singh Tomar with Irrfan Khan. It is the true story of an athlete who became a dacoit. In this, she played the title character's wife. Gill made her debut with Apoor
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Mahie Gill
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(1975-12-19) 19 December 1975 (age 49)Alma mater Panjab University Occupation Actor Years active 2003–present Spouse Ravi Kesar Children 1 Early life and education
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